Deglycosylation of Excretory-Secretory Antigens of the Second-Stage Larvae of Toxocara cati Improves Its Efficacy in the Diagnosis of Human Toxocariasis

Background. Toxocariasis is an important zoonotic infection, especially in tropical areas. One of the significant challenges serodiagnosis human toxocariasis cross-reaction Toxocara antigens with other parasites due to their relatively similar glycan structures. Removing structure from excretory-secretory (TES) may increase efficacy these diagnosis toxocariasis. The current study aimed assess deglycosylated cati (dTES) for Methods. ES were prepared T. second-stage larvae and using sodium hydroxide (NaOH). TES antigens, along dTES used ELISA as well a western blotting system detection anti-Toxocara antibodies. Sera samples collected 30 confirmed cases toxocariasis, patients diseases, healthy subjects evaluated by both systems. Results. sensitivity was 96.67% (95% CI = 82.78–99.92) 93.33% 77.93–99.18), respectively, while specificity (88.33%; 95% 77.43–95.18) increased significantly compared (80.00%; 67.67–89.22). 100% 88.43–100) system. Moreover, 86.08–98.96) 98.33% 91.06–99.96), when Conclusion. Results indicate that chemical removal epitopes reduces cross-reactivity rates parasitic infections. Considering findings present study, seem be suitable